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Updated: Mar 17, 2026

Preparation of Intact Tissue for Microscopic Analysis of the Endosperm Cell Layer in Developing and Mature Arabidopsis Seeds
Published on: May 16, 2025
Receptor-Mediated Endocytosis in Plant Cells
M. A. Horn1, P. F. Heinstein, P. S. Low
1Department of Chemistry, Purdue University, West Lafayette, Indiana 47907.
Abstract:
We have employed fluorescein and 125l-labeled elicitors of the defense response in soybeans to monitor the cellular distribution and movement of elicitors following their addition to a soybean cell suspension culture. Our results indicate that the macromolecular elicitors first bind to the cell surface and then internalize in a temperature- and energy-dependent endocytotic process. Within a few hours, virtually all of the elicitor is concentrated in the major vacuole or tonoplast of the cell. Nonspecific (control) proteins neither bound to the cell surface nor internalized in parallel assays.
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