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Updated: Sep 29, 2026
Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry (UPLC-HRMS)
Published on: May 20, 2013
Liquid chromatography-mass spectrometry method for determination of ramipril and its active metabolite ramiprilat in
Zhimeng Zhu1, Andre Vachareau, Len Neirinck
1Pharmascience, 6111 Royalmount Ave, Suite 100, Montreal, Quebec, H4P 2T4, Canada. zzhu@pharmascience.com
Abstract:
A fast and robust liquid chromatography-mass spectrometry (LC-MS-MS) method has been developed for simultaneous quantitation of the angiotensin-converting enzyme (ACE) inhibitor, ramipril and its metabolite ramiprilat in human plasma. The method involves a solid-phase extraction from plasma, simple isocratic chromatography conditions and mass spectrometric detection that enables a detection limit at sub-nanogram levels. The proposed method has been validated with a linear range of 0.5-250 ng/ml for both ramipril and ramiprilat. The overall recoveries for ramipril and ramiprilat were 88.7 and 101.8%, respectively.
