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Modified high-performance liquid chromatographic method for the determination of ertapenem in human urine: enhanced
Donald G Musson1, Chester J Kitchen, John Y-K Hsieh
1Merck Research Laboratories, WP75A-303, Sumneytown Pike, West Point, PA 19486, USA.
Abstract:
A column-switching, reversed-phase high-performance liquid chromatographic (HPLC) assay for a new structurally unique carbapenem antibiotic, ertapenem, in urine has been improved for selectivity and automated using a Packard MultiPROBE II EX pipetting station. The method uses column-switching for on-line extraction of the urine sample. The extraction column, analytical column, mobile phase, and timing of the column-switching valve have been changed to enhance selectivity for the analyte over endogenous background material. Sample transfer and dilution prior to direct-injection into the HPLC system have been accomplished using a Packard MultiPROBE II EX robotic liquid handling system.
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