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Imaging G Protein-coupled Receptor-mediated Chemotaxis and its Signaling Events in Neutrophil-like HL60 Cells
Published on: September 14, 2016
HL-60 cell growth-conditioned medium is an effective inducer of myeloperoxidase expression in K-562 human leukemia
Erich J Baker1, David A Gerard, Elena G Bamberger
1Department of Medical Genetics, University of Tennessee, Medical Center at Knoxville, Graduate School of Medicine, Box 2, 1924 Alcoa Highway, Knoxville, TN 37920, USA.
Abstract:
K-562 cells were cultured in HL-60 cell growth-conditioned medium (GCM) for up to 96h. Myeloperoxidase (MPO) mRNA was transiently detected by reverse transcription-polymerase chain reaction (RT-PCR) techniques at 12, 24, and 48h. The de novo expression of MPO protein was subsequently detectable by intracellular flow cytometry at 24, 48, 72 and 96h. Immunogold staining and cytochemical analysis demonstrated granularly-sequestered MPO in approximately 40% of HL-60 GCM-cultured cells after 48h of culture. The sequential detection of MPO mRNA and MPO biosynthesis is considered an indicator of serial maturation evocative of myeloblastic cells, and suggest that K-562 cells maintain the ability to differentiate along this lineage.
