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Determination of ethamsylate in pharmaceutical preparations based on an auto-oxidation chemiluminescence reaction
Fengzhen Yang1, Chao Zhang, Willy R G Baeyens
1Department of Chemistry, Tsinghua University, 100084 Beijing, People's Republic of China.
Abstract:
Strong chemiluminescence emission has been observed by mixing alkaline hydrolytic products of ethamsylate with Tween 80 in acidic rhodamine 6G solution. This phenomenon has been utilized to design a flow-injection chemiluminescence method for the determination of ethamsylate in a pharmaceutical preparation. Under the optimum conditions, the proposed procedure has a linear range between 0.05 and 2.0 microg ml(-1), with a detection limit of 0.02 microg ml(-1) for ethamsylate. The method was applied to the determination of ethamsylate in pharmaceutical preparations. The possible mechanism of this chemiluminescence reaction was proposed.
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