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Updated: Jul 20, 2026

Electricity-Free, Sequential Nucleic Acid and Protein Isolation
Published on: May 15, 2012
[RNA isolation and purification methods]
J P Bastard1, S Chambert, F Ceppa
1Service de biochimie et génétique moléculaire, Hôpital Cochin, 123, boulevard de Port-Royal, 75014 Paris, France.
Abstract:
Recent advances in human, bacterial and viral genome projects and the development of quantitative real-time reverse transcription-polymerase chain reaction methods offer the possibility of analysing a large number of gene transcripts. These molecular developments represent an important advancein the field of genetics, cancer, virology, bacteriology and hematology. A limiting step remains the isolation of high quality mRNA purified from biological samples. This review describes the different methods used to isolate mRNA from biological samples and to verify RNA integrity and gives precise details about RNA storage conditions.
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