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Related Experiment Videos

Respiration capacity and consequences in Lactococcus lactis.

Philippe Gaudu1, Karin Vido, Bénédicte Cesselin

  • 1Génétique Appliquée-URLGA, Institut National de la Recherche Agronomique, Domaine de Vilvert, 78352 Jouy en Josas, France.

Antonie Van Leeuwenhoek
|October 9, 2002
PubMed
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Lactococcus lactis can grow using respiration when heme is available, improving survival and biomass. This study suggests respiration, not fermentation, is optimal for lactococci growth and long-term survival.

Area of Science:

  • Microbiology
  • Bacterial Metabolism
  • Cellular Respiration

Background:

  • Lactococcus lactis is a well-studied fermenting bacterium.
  • Previous understanding suggested fermentation as the optimal growth condition for L. lactis.
  • Respiration in L. lactis was known to occur only when heme is present.

Purpose of the Study:

  • To investigate the metabolic changes and survival benefits of respirative growth in L. lactis.
  • To compare the growth and survival characteristics of L. lactis under fermentation versus respiration.
  • To elucidate the unique aspects of the L. lactis respiration model.

Main Methods:

  • Culturing L. lactis with and without heme under aerobic conditions.
  • Analyzing metabolic end products and biomass accumulation.

Related Experiment Videos

  • Assessing long-term survival rates of cells grown under different conditions.

    • Main Results:

    • Respirative growth of L. lactis, when supplemented with heme, doubles biomass and reduces acid production.
    • Cells grown via respiration exhibit significantly improved long-term survival compared to fermenting cells.
    • L. lactis initiates respiration late in growth due to the single cytochrome oxidase (bd) encoded by cydAB genes and the requirement for external heme.

    Conclusions:

    • Respiration-permissive conditions, rather than fermentation, appear optimal for L. lactis growth and survival.
    • Improved survival under respiration may be linked to reduced intracellular oxidation.
    • Related bacteria like Enterococci and some Lactobacilli with respiration potential may also benefit from respiration-permissive conditions.