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Related Experiment Videos

Elevated IgM anti-IgE in sera from allergic patients.

Olga Rudeschko1, Bärbel Fahlbusch, Maren Müller

  • 1Institute of Clinical Immunology, Friedrich Schiller University, Jena, Germany. orud@mti-n.uni-jena.de

Journal of Investigational Allergology & Clinical Immunology
|October 10, 2002
PubMed
Summary

This study found higher levels of IgM anti-IgE autoantibodies in allergic patients, but not IgG anti-IgE. IgE/IgM complexes are present in blood, while IgE/IgG complexes may form during testing.

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Area of Science:

  • Immunology
  • Allergy Research
  • Autoantibody Studies

Background:

  • The role of anti-IgE autoantibodies in IgE-related allergic diseases is unclear, with conflicting reports on their effects.
  • Previous studies have shown varied correlations between total IgE and IgE/IgG complexes.

Purpose of the Study:

  • To investigate the role of anti-IgE autoantibodies in allergic diseases.
  • To compare IgE levels and anti-IgE autoantibody profiles in allergic patients versus controls and rheumatoid arthritis patients.

Main Methods:

  • Enzyme-linked immunosorbent assay (ELISA) to detect free and complexed IgG and IgM anti-IgE.
  • Superose 6 column separation to determine IgE/IgG and IgE/IgM complex levels.

Main Results:

Related Experiment Videos

  • Allergic patients showed significantly higher free IgM anti-IgE levels compared to controls and rheumatoid arthritis patients.
  • IgE/IgM complexes also tended to be higher in allergic patients, while IgG anti-IgE and IgE/IgG complexes did not differ significantly.
  • IgE/IgM complexes were detected at higher molecular weights (330 kDa) than IgE/IgG complexes (150-180 kDa).

Conclusions:

  • Increased IgM anti-IgE autoantibody titers in allergic patients are not correlated with high total IgE levels.
  • IgE/IgG complexes likely form during ELISA, whereas IgE/IgM complexes are presumed to exist in circulating blood.