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Related Experiment Videos

B plexins activate Rho through PDZ-RhoGEF.

Mariette H E Driessens1, Cristina Olivo, Koh-ichi Nagata

  • 1Division of Cell Biology, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands. mariette@niob.knaw.nl

FEBS Letters
|October 10, 2002
PubMed
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Plexin-B1 receptors bind semaphorins and activate Rho signaling. This interaction, mediated by PDZ-RhoGEF, is crucial for stress fiber formation and cell migration during axon guidance.

Area of Science:

  • Cell biology
  • Neuroscience
  • Molecular signaling

Background:

  • Plexins function as semaphorin receptors, mediating repulsive axon guidance.
  • Plexin-B1 was previously shown to bind activated Rac, but clustering induces Rho activation and stress fiber formation.

Purpose of the Study:

  • To investigate the molecular mechanism by which plexin-B1 clustering activates Rho signaling.
  • To identify the specific proteins involved in mediating plexin-B1-induced Rho activation.

Main Methods:

  • Yeast two-hybrid system to identify protein-protein interactions.
  • Site-directed mutagenesis of plexin-B1 carboxy-terminal residues.
  • Coexpression of dominant-negative PDZ-RhoGEF.

Main Results:

Related Experiment Videos

  • The C-terminus of B plexins directly interacts with Rho-specific guanine nucleotide exchange factors (GEFs) via their PDZ domain.
  • Mutation of plexin-B1's C-terminus abrogated stress fiber formation.
  • Dominant-negative PDZ-RhoGEF blocked plexin-B1-induced stress fiber formation.

Conclusions:

  • PDZ-RhoGEF is a key mediator in B plexin-induced Rho/Rho kinase signaling.
  • This signaling pathway is implicated in regulating axon guidance and cell migration.