Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Heme oxygenase-1 localization in the rat nephron.

Michelle Shepard1, Prasad Dhulipala, Sunit Kabaria

  • 1Department of Medicine, Nephrology Division, Robert Wood Johnson Medical School, New Brunswick, NJ 08903, USA.

Nephron
|October 10, 2002
PubMed
Summary

Heme oxygenase-1 (HO-1) is expressed in rat kidney's distal tubules and collecting ducts. This suggests these segments are protected against oxidative injury in nephropathies.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Clinical screening and peer interventions to prevent substance use in at-risk adolescents: A longitudinal single-arm trial protocol.

Contemporary clinical trials communications·2026
Same author

RETRACTED: Shen et al. Cold-Pressed <i>Nigella Sativa</i> Oil Standardized to 3% Thymoquinone Potentiates Omega-3 Protection against Obesity-Induced Oxidative Stress, Inflammation, and Markers of Insulin Resistance Accompanied with Conversion of White to Beige Fat in Mice. <i>Antioxidants</i> 2020, <i>9</i>, 489.

Antioxidants (Basel, Switzerland)·2025
Same author

Constitutive HO-1 and CD55 (DAF) Expression and Regulatory Interaction in Cultured Podocytes.

Biomedicines·2023
Same author

Metalloporphyrins Reduce Proteinuria in Podocyte Immune Injury: The Role of Metal and Porphyrin Moieties.

International journal of molecular sciences·2023
Same author

Immune-Related Functions of Heme Oxygenase-1.

Antioxidants (Basel, Switzerland)·2023
Same author

Metalloporphyrins as Tools for Deciphering the Role of Heme Oxygenase in Renal Immune Injury.

International journal of molecular sciences·2023

Area of Science:

  • Nephrology
  • Molecular Biology
  • Biochemistry

Background:

  • Renal tubules are susceptible to oxidative injury in various kidney diseases.
  • The protective mechanisms within tubular cells against oxidative damage are not fully understood.
  • Heme oxygenase-1 (HO-1) is a key enzyme in heme catabolism, potentially reducing oxidative stress by lowering free heme levels.

Purpose of the Study:

  • To determine the distribution of HO-1 within the nephron.
  • To identify specific tubular segments where HO-1 expression may offer protection against oxidative injury.

Main Methods:

  • Rats were administered HO-1 inducers (stannous chloride or cobalt protoporphyrin).
  • Kidney sections were stained for HO-1 using immunohistochemistry.
  • Lectins (Tetragonolobus purpureas and Arachnis hypogaea) were used to identify proximal versus distal/collecting tubules.

Related Experiment Videos

Main Results:

  • HO-1 was localized to tubules, but not glomeruli, in induced rat kidneys.
  • Proximal tubules showed negative staining for HO-1.
  • Distal tubules and collecting ducts exhibited positive HO-1 staining.

Conclusions:

  • The distal nephron, including distal tubules and collecting ducts, is the primary site of HO-1 expression in the rat kidney following induction.
  • HO-1 expression in the distal nephron may confer resistance to oxidative injury in kidney diseases.