Jove
Visualize
Contact Us

Related Experiment Videos

Escherichia coli insertion sequence IS150: transposition via circular and linear intermediates.

Markus Haas1, Bodo Rak

  • 1Institut für Biologie III, Universität, Schänzlestrasse 1, D-79104 Freiburg, Germany.

Journal of Bacteriology
|October 11, 2002
PubMed
Summary

The IS150 transposase (InsAB) drives transposition, creating circular and linear DNA intermediates. InsA protein prevents premature linear product formation, ensuring efficient DNA insertion.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The association between acute otitis externa-related emergency department visits and extreme weather events in a temperate continental climate.

International journal of hygiene and environmental health·2024
Same author

Changes in management of head and neck malignancies during the COVID-19 pandemic.

Acta otorhinolaryngologica Italica : organo ufficiale della Societa italiana di otorinolaringologia e chirurgia cervico-facciale·2024
Same author

Circulating Tumor DNA in the Immediate Postoperative Setting.

Annals of surgical oncology·2024
Same author

Presentation Rates for Acute Pharyngitis in the Emergency Room Are Influenced by Extreme Weather Events.

Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery·2023
Same author

The ATR inhibitor berzosertib acts as a radio- and chemosensitizer in head and neck squamous cell carcinoma cell lines.

Investigational new drugs·2023
Same author

Immunohistochemical Assessment of Microvessel Density in OSCC: Spatial Heterogeneity of Angiogenesis and Its Impact on Survival.

Biomedicines·2023
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Area of Science:

  • Molecular Biology
  • Genetics
  • Microbiology

Background:

  • IS150 is a member of the IS3 family of mobile genetic elements.
  • IS elements transpose via DNA intermediates, but their precise nature and regulation remain under investigation.
  • IS3 family elements encode proteins via overlapping open reading frames and programmed translational frameshifting.

Purpose of the Study:

  • To identify the transposase of the IS150 element.
  • To characterize the transposition intermediates generated by IS150 in vivo.
  • To elucidate the role of the InsA protein in IS150 transposition.

Main Methods:

  • Analysis of IS150 open reading frames and protein products.
  • In vivo generation and characterization of IS150 transposition intermediates (circular and linear DNA molecules).

Related Experiment Videos

  • Site-directed mutagenesis to eliminate InsA synthesis and assess its impact on transposition.

    • Main Results:

    • The InsAB protein was identified as the IS150 transposase.
    • Wild-type IS150 generated circular and linear DNA molecules in vivo, consistent with transposition intermediates.
    • Circular forms appear to be precursors to linear molecules.
    • Elimination of InsA synthesis led to an accumulation of linear IS150 molecules.

    Conclusions:

    • The InsAB protein is the functional transposase for IS150.
    • Circular and linear IS150 molecules represent true transposition intermediates.
    • InsA plays a crucial role in regulating transposition, likely by preventing abortive production of linear intermediates and coupling their generation to productive insertion events.