Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Euchromatinization of mammalian nuclei.

G T Matioli1

  • 1USC Medical School, California, Los Angeles 90033, USA.

Medical Hypotheses
|October 12, 2002
PubMed
Summary

Internal vesicles in mammalian nuclei disassemble, converting heterochromatin into euchromatin. This mechanism drives changes in chromatin structure and nuclear organization.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Homotopic dynamics of telomeric loops in mammalian chromatids.

Journal of theoretical biology·2007
Same author

Chromatid transport by pantographic motors (PMS).

Medical hypotheses·2003
Same author

BCR-ABL insufficiency for the transformation of human stem cells into CML.

Medical hypotheses·2002
Same author

A note on the meaning of stochasticity.

Medical hypotheses·2002
Same author

On metastatic seeding of regional lymph nodes.

Medical hypotheses·2002
Same author

Go and chemoresistance of malignant cells.

Medical hypotheses·2002

Area of Science:

  • Cell Biology
  • Genetics
  • Epigenetics

Background:

  • Chromatin structure, comprising heterochromatin and euchromatin, dictates gene accessibility.
  • Mammalian nuclei contain internal vesicles whose function and fate are not fully understood.

Purpose of the Study:

  • To elucidate a novel mechanism for heterochromatin to euchromatin conversion.
  • To investigate the role of internal vesicles in nuclear state transitions.

Main Methods:

  • Microscopy techniques to observe vesicle dynamics within the nucleus.
  • Biochemical assays to analyze vesicle composition and disassembly.
  • Chromatin immunoprecipitation to assess heterochromatin and euchromatin states.

Main Results:

  • Observed disassembly of internal vesicles within the mammalian nucleus.
  • Demonstrated a correlation between vesicle disassembly and the conversion of heterochromatin to euchromatin.
  • Showed that vesicles cannot survive when trapped in the nuclear chamber, triggering their breakdown.

Conclusions:

  • Vesicle disassembly is a key mechanism driving the transition from heterochromatin to euchromatin.
  • This process influences nuclear organization and potentially gene regulation.
  • The findings offer new insights into the dynamic nature of chromatin remodeling.

Related Experiment Videos