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Screening of single nucleotide polymorphisms in nasopharyngeal carcinoma associated genes by denaturing
Hua Huang1, Yu-Feng Zhou, Pei-Zhou Jiang
1Institute of Oncology, First Military Medical University, Guangzhou 510515, China.
Objective:
To screen single nucleotide polymorphisms (SNPs) of 4 human genes at 6p21.3 by way of denaturing high-performance liquid chromatography (DHPLC).
Method:
Four exons and 1 intron fragments in the PPP1R11, PPP1R10, FLOT1 and KIAA0170 genes at 6p21.3 isolated from the blood samples from 30 patients with nasopharyngeal carcinoma (NPC) and 28 healthy subjects were amplified by PCR, and the products analyzed by DHPLC. Some fragments of interest were sequenced and compared with the sequences available in National Center for Biotechnology Information (NCBI) database.
Results:
In the 5 fragments, 4 new SNPs were identified and 4 known SNP loci and genotypes were confirmed.
Conclusion:
DHPLC is an effective, economical, and simple method with reliability for SNPs screening.
