Related Experiment Video
Updated: Jul 17, 2026

A Robotic Platform for High-throughput Protoplast Isolation and Transformation
Published on: September 27, 2016
Alternative methods of plant transformation--a short review
1Department of Plant Genetics, Breeding and Biotechnology, Warsaw Agricultural University, Nowoursynowska 166, 02-787 Warszawa, Poland. RAKOCZY@ALPHA.SGGW.WAW.PL
Abstract:
Several methods of transformation are currently available for delivering exogenous DNA to plant cells. Agrobacterium-mediated transformation, microprojectile bombardment and direct protoplast transformation are routinely used today. However, each of them has certain disadvantages, which led to research into the development of novel alternative systems such as infiltration, electroporation of cells and tissues, electrophoresis of embryos, microinjection, pollen-tube pathway, silicon carbide- and liposome-mediated transformation. The low efficiency of transformation is considered to be the main reason for the limited popularity of the alternative transformation methods, other than infiltration and silicon carbide-mediated transformation, which seem to be the most promising ones for practice.
More Related Videos
Related Concept Videos
Transgenic Organisms
Plant Tissue Culture
Plant Breeding and Biotechnology
Transgenic Plants
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
Transformation

