Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Reusable platforms for high-throughput on-chip temperature gradient assays.

Hanbin Mao1, Matthew A Holden, Min You

  • 1Department of Chemistry, Texas A&M University, College Station 77843, USA.

Analytical Chemistry
|October 17, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Conceptualization, Contexts, and Measurement of Nursing Theoretical Literacy: Protocol for a Scoping Review.

JMIR research protocols·2026
Same author

Lipid-Substrate Interactions Lead to Bilayer Asymmetry.

Journal of the American Chemical Society·2026
Same author

Conceptualization, Contexts, and Measurement of Nursing Theoretical Literacy: Protocol for a Scoping Review.

JMIR research protocols·2026
Same author

Mechanozyme: An Artificial Enzyme With a Mechanophore Framework.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Attitudes and participation in fall risk management: A tripartite, multicenter cross-sectional study of physicians, nurses, and patients.

PloS one·2026
Same author

Nucleation dynamics in amyloid-beta dimerization revealed by single-molecule fingerprinting.

Cell reports. Physical science·2026
Same journal

Modeling the Effects of Short-Range Randomness in Packed Sphere Beds.

Analytical chemistry·2026
Same journal

Mitochondrial Redox Cascade-Directed Covalent NIR Fluorogenic Imaging of Therapy-Induced Senescence Integrates Tumor and Host Responses.

Analytical chemistry·2026
Same journal

Proteomic Profiling of RHD-Related Mitral Annulus Calcification Enabled by Magnetic Carbon Nanomaterial-Supported Quasi-Immobilized Enzyme Digestion.

Analytical chemistry·2026
Same journal

Spatial-Photonic Encoding on a Single Fiber: Breaking the Bottleneck in Photoelectrochemical Biosensing for Precision Diagnostics.

Analytical chemistry·2026
Same journal

Spreadable Biosensing Pregel for Analyte Visualization in Peeled Plant Tissues.

Analytical chemistry·2026
Same journal

DARibo-Q: RNA Allosteric Transduction for Fluorescence Imaging of Dopamine Modulation in Living Systems.

Analytical chemistry·2026
See all related articles

This study presents a novel platform for applying linear temperature gradients to microfluidic devices, enabling simultaneous, high-throughput data collection for chemical and biochemical analyses. The system efficiently distinguishes DNA sequences and measures fluorescein dye properties across varying conditions.

Area of Science:

  • Biotechnology
  • Analytical Chemistry
  • Materials Science

Background:

  • Lab-on-a-chip devices offer miniaturized platforms for complex analyses.
  • Precise temperature control is crucial for many biochemical reactions and assays.
  • Concurrent data acquisition can significantly accelerate experimental throughput.

Purpose of the Study:

  • To develop and demonstrate a reusable platform for applying linear temperature gradients to microfluidic devices.
  • To enable concurrent, temperature-dependent data collection for chemical and biochemical species.
  • To showcase the platform's capability for high-throughput, multi-variable experiments.

Main Methods:

  • A reusable platform was designed to apply a linear temperature gradient across a planar microfluidic device.

Related Experiment Videos

  • Microchannels were oriented perpendicular to the gradient for discrete temperature control.
  • An alternative configuration allowed for a continuous temperature gradient parallel to microchannel flow.
  • Experiments included dsDNA melting curve analysis and fluorescein fluorescence yield measurement.

    • Main Results:

    • The platform successfully applied stable linear temperature gradients, allowing discrete temperature control across microchannels.
    • Simultaneous dsDNA melting curve analysis distinguished perfect matches from single-nucleotide mismatches.
    • Concurrent measurement of fluorescein fluorescence demonstrated high-throughput analysis across concentration and temperature variables.

    Conclusions:

    • The developed platform enables efficient, concurrent, temperature-dependent data acquisition in microfluidic systems.
    • This technology facilitates high-throughput screening and analysis of chemical and biochemical processes.
    • The platform's versatility supports various applications, including molecular diagnostics and chemical sensing.