Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Deregulated G1-cyclin expression induces genomic instability by preventing efficient pre-RC formation.

Seiji Tanaka1, John F X Diffley

  • 1Cancer Research UK, Clare Hall Laboratories, South Mimms, Herts EN6 3LD, UK.

Genes & Development
|October 17, 2002
PubMed
Summary

Overexpression of the G1 cyclin Cln2 in yeast inhibits DNA replication complex assembly, leading to genomic instability and chromosome rearrangements. This suggests a mechanism linking cell cycle deregulation to cancer development.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Structure of the pre-initiation complex explains CMGE biogenesis.

Nature·2026
Same author

Targeting cancer-specific mutations with RNA-triggered chromatin shredding.

Nature·2026
Same author

Selective Elimination of TP53 Mutant Cells by Transcript-Activated Chromatin Shredding.

bioRxiv : the preprint server for biology·2026
Same author

Multicenter Retrospective Analysis of Acute Scrotum in the Kyushu-Okinawa Region: Diagnostic Practices, Management Patterns, and Predictors of Testicular Salvage.

International journal of urology : official journal of the Japanese Urological Association·2025
Same author

Impact of Trunk Versus Skeletal Muscle Mass Gain on Balance Improvement in Patients with Cerebral Infarction.

JMA journal·2025
Same author

Rituximab-induced long-term remission in childhood-onset, uncomplicated, frequently relapsing or steroid-dependent nephrotic syndrome: a randomized, placebo-controlled trial and a follow-up study.

Scientific reports·2025

Area of Science:

  • Molecular Biology
  • Cell Cycle Regulation
  • Cancer Genomics

Background:

  • Genomic instability is a key feature of human cancers, but its origins are unclear.
  • Deregulation of G1 cyclins is common in human cancers.
  • The link between cell cycle control and the generation of genomic instability is not fully understood.

Purpose of the Study:

  • To investigate how deregulation of G1 cyclins contributes to genomic instability.
  • To explore the mechanisms underlying the generation and selection of genomic instability during oncogenesis.
  • To elucidate the role of G1 cyclin deregulation in inhibiting replication licensing.

Main Methods:

  • Utilized budding yeast (Saccharomyces cerevisiae) as a model organism.
  • Studied the effects of overexpressing the G1 cyclin Cln2.

Related Experiment Videos

  • Assessed the impact on prereplicative complex (pre-RC) assembly and gross chromosome rearrangements (GCRs).

    • Main Results:

    • Overexpression of Cln2 was shown to inhibit the assembly of prereplicative complexes (pre-RCs).
    • Cln2 overexpression induced gross chromosome rearrangements (GCRs) in yeast cells.
    • The study identified a direct link between G1 cyclin deregulation and the inhibition of replication licensing.

    Conclusions:

    • Deregulation of G1 cyclins, potentially selected for during oncogenesis due to growth advantages, can generate genomic instability.
    • Inhibition of replication licensing by deregulated G1 cyclins is a proposed mechanism for generating genomic instability.
    • Findings in yeast provide insights into mechanisms relevant to human cancer development.