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A requirement for reversible binding between aggregating embryonic cells before stable adhesion.

J Umbreit, S Roseman

    The Journal of Biological Chemistry
    |December 25, 1975
    PubMed
    Summary
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    Intercellular adhesion.

    Upsala journal of medical sciences·2010

    Chick embryonic cells aggregate in two steps: first a reversible, energy-independent binding, then a stable, energy-dependent attachment. This process is crucial for tissue formation.

    Area of Science:

    • Developmental Biology
    • Cell Biology
    • Tissue Engineering

    Background:

    • Cell aggregation is fundamental for embryonic development and tissue formation.
    • Understanding the mechanisms of cell-cell binding is key to developmental processes.

    Purpose of the Study:

    • To elucidate the two-step mechanism of cell aggregation in chick embryonic liver and neural retina cells.
    • To investigate the energy requirements and reversibility of initial cell-cell interactions.

    Main Methods:

    • Observed cell aggregation in chick embryonic liver and neural retina cell cultures.
    • Assessed energy dependence using metabolic inhibitors (implied).
    • Evaluated bond reversibility through shear force and dilution experiments.
    • Conducted kinetic studies to determine the sequence of binding events.

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  • Examined temperature dependence of bond formation.

    • Main Results:

    • A two-step aggregation process was identified: initial loose association followed by stable attachment.
    • The initial association was reversible, energy-independent, and sensitive to mild shear forces or dilution.
    • Stable attachment required metabolic energy and was irreversible.
    • Reversible binding preceded and was necessary for stable binding.
    • Both binding steps were temperature-dependent.

    Conclusions:

    • Chick embryonic cell aggregation involves a sequential process of reversible and stable binding.
    • The initial reversible step is a prerequisite for the subsequent energy-dependent stable attachment.
    • This two-step mechanism provides a framework for understanding tissue morphogenesis.