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CD14+,CD16+ blood monocytes and joint inflammation in rheumatoid arthritis

Norikuni Kawanaka1, Masahiro Yamamura, Tetsushi Aita

  • 1Department of Medicine and Clinical Science, Graduate School of Medicine and Dentistry, Okayama University, 2-5-1 Shikata-cho, Okayama 700-8558, Japan.

Arthritis and Rheumatism
|October 18, 2002
PubMed
Abstract

Insights

CD14+,CD16+ monocytes are elevated in rheumatoid arthritis (RA) patients, correlating with disease activity. Cytokine-induced maturation of these monocytes may drive persistent joint inflammation in RA.

Area of Science:

  • Immunology
  • Rheumatology

Background:

  • CD14+,CD16+ monocytes are a minor subset in human peripheral blood (PB).
  • This monocyte subset has been implicated in various inflammatory diseases.

Purpose of the Study:

  • To investigate the role of CD14+,CD16+ monocytes in joint inflammation in rheumatoid arthritis (RA).

Main Methods:

  • Flow cytometry measured CD14, CD16, CCR1, CCR5, and ICAM-1 expression on monocytes.
  • ELISA measured cytokine concentrations (TGF-beta1, M-CSF, IL-10) and soluble CD14 (sCD14).
  • In vitro culture assessed CD16 induction on RA monocytes by cytokines.

Main Results:

  • RA patients showed a significantly higher frequency of CD14+,CD16+ blood monocytes compared to healthy controls.
  • Increased CD16+ monocyte frequency correlated with active RA disease markers and higher cytokine/sCD14 levels.
  • CD14+,CD16+ monocytes expressed higher levels of CCR1, CCR5, and ICAM-1, particularly in active RA.

Conclusions:

  • Elevated CD14+,CD16+ monocytes in RA patients suggest their involvement in disease pathogenesis.
  • Cytokine-induced maturation of monocytes before joint entry may contribute to persistent RA joint inflammation.

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