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Updated: Jul 29, 2026

Determination of the Relative Potency of an Anti-TNF Monoclonal Antibody (mAb) by Neutralizing TNF Using an In Vitro Bioanalytical Method
Published on: September 16, 2017
Antitumour necrosis factor therapy in Crohn's disease
1Department of Medicine, University Hospital Innsbruck, Anichstrasse 35, 6020 Innsbruck, Austria. herbert.tilg@uibk.ac.at
Abstract:
The key role and contribution of various pro-inflammatory cytokines in common chronic inflammatory disorders such as inflammatory bowel disease (IBD) has been thoroughly investigated in recent years. Besides IL-1, TNF-alpha has been identified as one of the central immune mediators controlling inflammatory processes. Not surprisingly, neutralisation of these cytokines has been introduced into clinical research. Crohn's disease (CD) is one of the chronic inflammatory disorders where TNF-alpha seems to have a particularly important role. Neutralisation of this cytokine by specific antibodies, for example infliximab, has been shown recently to affect the clinical phenotype of this disorder. Other TNF-neutralising approaches such as etanercept have also been studied in patients with CD but with less successful outcomes. These new cytokine-targeting approaches have changed clinical medicine in the field of inflammatory disorders.
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