Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Endothelial cells maintain a reduced redox environment even as mitochondrial function declines.

Ricarda Carlisle1, Carol Ann Rhoads, Tak Yee Aw

  • 1Department of Molecular and Cellular Physiology, Louisiana Health Sciences Center, Shreveport, Louisiana 71130, USA.

American Journal of Physiology. Cell Physiology
|October 22, 2002
PubMed
Summary

Human umbilical vein endothelial cells (HUVECs) undergo genomic instability and mitochondrial dysfunction with replication. Despite this, they maintain a reducing environment, crucial for cellular health in aging vascular disease.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Outcomes of Germline Testing for Children with Hematologic Malignancies Undergoing Hematopoietic Cell Transplantation.

Blood advances·2026
Same author

Expanding frontiers: harnessing plant biology for space exploration and planetary sustainability.

The New phytologist·2025
Same author

Investigation of DNA Damage Response Genes Validates the Role of DNA Repair in Pediatric Cancer Risk and Identifies <i>SMARCAL1</i> as a Novel Osteosarcoma Predisposition Gene.

Journal of clinical oncology : official journal of the American Society of Clinical Oncology·2025
Same author

Contribution of Germline Predisposition to Pediatric Thyroid Cancer.

Clinical cancer research : an official journal of the American Association for Cancer Research·2025
Same author

Comprehensive investigation of DNA damage repair genes in children with cancer identifies <i>SMARCAL1</i> as novel osteosarcoma predisposition gene.

medRxiv : the preprint server for health sciences·2025
Same author

The moderating role of parental cognitive perceptions in the link between children's cancer predisposition genetic testing results and parent psychological adjustment.

Journal of pediatric psychology·2025

Area of Science:

  • Cell Biology
  • Mitochondrial Biology
  • Gerontology

Background:

  • Replicative senescence in human umbilical vein endothelial cells (HUVECs) is linked to oxidative stress and mitochondrial dysfunction.
  • These factors are implicated in the pathogenesis of atherosclerosis, an age-related vascular disease.

Purpose of the Study:

  • To investigate the impact of cell division on genomic stability, mitochondrial function, and redox status in HUVECs.
  • To understand cellular adaptations during replicative senescence.

Main Methods:

  • Culturing HUVECs to approximately 60 cumulative population doublings (CPD).
  • Assessing nuclear genome integrity, cell size, oxygen consumption, ATP production, and mitochondrial mass.
  • Measuring mitochondrial genome copy number and antioxidant capacity (reduced glutathione, glutathione peroxidase, GSSG reductase, glucose-6-phosphate dehydrogenase).

Related Experiment Videos

Main Results:

  • After 20 CPD, HUVECs showed nuclear genome deterioration, increased cell size, oxygen consumption, ATP production, and mitochondrial mass.
  • Antioxidant capacity initially increased, but by CPD 52, key enzymes and mitochondrial function markers declined.
  • Despite functional decline, HUVECs maintained a highly reducing environment.

Conclusions:

  • HUVECs exhibit genomic instability and declining mitochondrial function during replicative senescence.
  • Cellular adaptations allow HUVECs to preserve a reducing intracellular environment despite aging.
  • These findings offer insights into cellular mechanisms relevant to vascular aging and disease.