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[Effects of lipofectin-mediated alpha1,4Gal T antisense oligonucleotide on human glioma cell line SWO-38]
Gang Zheng1, Ji-Ren Zhang, Fa-Quan Liu
1Department of Oncology, Zhujiang Hospital, First Military Medical University, Guangzhou 510282, China.
Objective:
To study the effects of alpha1,4Gal T antisense oligonucleotide mediated by lipofectin on human glioma cell line SWO-38.
Methods:
SWO38 glioma cells were exposed to 10 micromol/L alpha1,4Gal T antisense oligonucleotide for 72 h by means of lipofectin transfection, and the growth inhibition of the cells was detected by colony-forming unit assay. Analysis of DNA fragmentation was performed with flow cytometry (FCM) and agarose gel eletrophoresis with Fas protein expression determined by FCM.
Results:
alpha1,4Gal T antisense oligonucleotide significantly inhibited the growth of glioma cells (P<0.01) and induced apoptosis in SWO-38 cells (P<0.05). Marked up-regulation of Fas protein expression was observed in response to the treatment (P<0.01).
Conclusion:
alpha1,4Gal T antisense oligonucleotide can significantly inhibit SWO-38 cell proliferation and induce cellular apoptosis, the mechanism of which may involve up-regulated Fas expression.
Insights
Alpha1,4Gal T antisense oligonucleotide significantly inhibited human glioma cell growth and induced apoptosis. This effect may be linked to increased Fas protein expression in SWO-38 cells.
Area of Science:
- Molecular Biology
- Cancer Research
- Cell Biology
Background:
- Glioma is a primary brain tumor with limited treatment options.
- Antisense oligonucleotides offer a targeted therapeutic approach.
- Understanding molecular mechanisms of glioma growth is crucial for developing new therapies.
Purpose of the Study:
- To investigate the impact of alpha1,4Gal T antisense oligonucleotide on human glioma cell line SWO-38.
- To determine if lipofectin-mediated delivery of the oligonucleotide affects cell proliferation and apoptosis.
- To explore the role of Fas protein expression in the observed cellular responses.
Main Methods:
- SWO-38 human glioma cells were treated with alpha1,4Gal T antisense oligonucleotide using lipofectin.
- Cell growth inhibition was assessed via colony-forming unit assay.
- Apoptosis and Fas protein expression were analyzed using flow cytometry and agarose gel electrophoresis.
Main Results:
- Alpha1,4Gal T antisense oligonucleotide significantly inhibited SWO-38 glioma cell proliferation (P<0.01).
- The treatment induced significant apoptosis in SWO-38 cells (P<0.05).
- A marked up-regulation of Fas protein expression was observed post-treatment (P<0.01).
Conclusions:
- Alpha1,4Gal T antisense oligonucleotide effectively inhibits proliferation of SWO-38 human glioma cells.
- The oligonucleotide induces apoptosis in these cells, suggesting a potential anti-cancer effect.
- Up-regulation of Fas protein expression is a likely mechanism underlying the observed anti-proliferative and apoptotic effects.