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Characterization of the murine CD30 ligand (CD153) gene: gene structure and expression
L C Goldie-Cregan1, E J Croager, L J Abraham
1The University of Western Australia, Crawley.
Abstract:
CD153 (CD30 ligand) has been described as a 40-kDa type II transmembrane glycoprotein belonging to the TNF superfamily and is expressed primarily by activated T cells, B cells and monocytes. In this study, we have determined that the murine CD153 gene consists of four exons, with three intervening introns, spaced over approximately 26 kb of genomic sequence. Sequence analysis of the murine CD153 promoter and 5' flanking region revealed the presence of a TATA box element immediately upstream of two tsp sites, together with putative binding motifs for a variety of lymphoid-specific transcription factors. 5'RACE analysis of LPS-stimulated RAW264.7 macrophage cDNA identified at least four transcriptional start sites for murine CD153, with two sites occurring downstream of the previously predicted translation initiation codon. Additionally, 5' RACE analysis identified multiple murine CD153 polyadenylation sites. Our results indicate that primary murine CD153 transcripts may vary from 26 kb to approximately 28 kb in length.
Insights
This study details the murine CD153 gene structure, revealing its four exons and promoter region. We identified multiple transcriptional start and polyadenylation sites, influencing CD153 transcript length.
Area of Science:
- Immunology
- Molecular Biology
- Genomics
Background:
- CD153, also known as CD30 ligand, is a type II transmembrane glycoprotein within the TNF superfamily.
- It is primarily expressed on activated T cells, B cells, and monocytes.
Purpose of the Study:
- To elucidate the genomic structure of the murine CD153 gene.
- To identify regulatory elements and transcriptional start sites of murine CD153.
Main Methods:
- Genomic sequence analysis of the murine CD153 gene.
- Sequence analysis of the promoter and 5' flanking region.
- 5' RACE (Rapid Amplification of cDNA Ends) analysis on LPS-stimulated macrophage cDNA.
Main Results:
- The murine CD153 gene comprises four exons spanning approximately 26 kb.
- Analysis revealed a TATA box and putative lymphoid-specific transcription factor binding motifs in the promoter region.
- Multiple transcriptional start sites and polyadenylation sites were identified, suggesting transcript length variation from 26 kb to 28 kb.
Conclusions:
- The murine CD153 gene structure and regulatory elements provide insight into its expression control.
- The identified transcriptional and polyadenylation sites contribute to the heterogeneity of primary CD153 transcripts.