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Lysosomes in the cultivated mushroom (Psalliota bispora quel.)
Abstract:
Fungine lysosomes are difficult to identify because some vesicular structures are present in hyphae, such as provacuoles, lomasome precursor, peroxisomes and spherosomes. Moreover, many Hymenomycetes, as the common cultivated mushroom (Psalliota bispora Quel.), lack tipical Golgi apparatuses, making ontogenesis of lysosomes a matter of difficult interpretation. As shown before (Scannerini, 1969) the hyphae of this fungus contain vesicles that reveal acid glycerophosphatase activity, thus we studied these organelles with morphological, cytochemical and biochemical criteria.
Insights
Identifying fungal lysosomes is challenging due to similar vesicular structures and the absence of typical Golgi apparatus in many fungi. This study investigated these organelles using multiple methods to clarify their nature.
Area of Science:
- Mycology
- Cell Biology
- Biochemistry
Background:
- Fungal lysosomes are difficult to identify due to the presence of similar vesicular structures in hyphae.
- Many Hymenomycetes, like the cultivated mushroom (Psalliota bispora), lack typical Golgi apparatus, complicating lysosome ontogenesis interpretation.
Purpose of the Study:
- To investigate the nature of vesicular structures in fungal hyphae exhibiting acid glycerophosphatase activity.
- To overcome identification challenges of fungal lysosomes in Hymenomycetes.
Main Methods:
- Morphological analysis of fungal hyphae.
- Cytochemical staining for acid glycerophosphatase activity.
- Biochemical assays to characterize organelles.
Main Results:
- Vesicles with acid glycerophosphatase activity were identified in fungal hyphae.
- Morphological, cytochemical, and biochemical data were used to characterize these organelles.
Conclusions:
- The study provides criteria for identifying fungal lysosomes, particularly in Hymenomycetes.
- Understanding lysosome identification is crucial for fungal cell biology research.