A new collagen from the extracellular matrix of Sepia officinalis cartilage
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Updated: Sep 28, 2026

Fabrication of Decellularized Cartilage-derived Matrix Scaffolds
Published on: January 7, 2019
Cristina Rigo1, Aurelio Bairati
1Dipartimento di Fisiologia e Biochimica Generali, Sezione di Istologia ed Anatomia Umana, Università degli Studi di Milano, via Celoria 26, 20133 Milan, Italy. rigo@mailserver.unimi.it
Abstract:
Guanidinium chloride treatment of Sepia officinalis cartilage solubilized a component that contained hydroxyproline. Electron-microscopy observation of rotary-shadowed preparations of this component revealed it to consist of rod-like units themselves consisting of filaments. Dialysis of an acetic acid solution against ATP afforded polymeric aggregates consisting of a succession of two or three thick sections showing transverse electron-opaque banding, separated by thinner sections without banding. Electrophoresis produced a main band of about 140 kDa sensitive to bacterial collagenase. After reduction with mercaptoethanol, electrophoresis afforded a 40-kDa band. Pepsin digestion resulted in additional electrophoretic bands. These data suggest the presence of a collagen in Sepia cartilage with characteristics unlike those of any known collagen.
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