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A rapid assay for icodextrin determination in plasma and dialysate
Run Wang1, James B Moberly, Leo Martis
1Baxter Healthcare Corporation, Renal Division, McGaw Park, Illinois, USA.
Advances in Peritoneal Dialysis. Conference on Peritoneal Dialysis
|October 31, 2002
Summary
A new assay simplifies icodextrin measurement in peritoneal dialysis patients. This method uses amyloglucosidase to accurately determine icodextrin levels in plasma and dialysate, improving patient monitoring.
Area of Science:
- Biochemistry
- Clinical Chemistry
- Nephrology
Background:
- Icodextrin is crucial for sustained ultrafiltration in peritoneal dialysis.
- Traditional icodextrin assays are time-consuming and complex.
- A need exists for a more efficient and reliable method.
Purpose of the Study:
- To develop and validate a simplified assay for icodextrin in biological samples.
- To enable faster and more routine analysis of icodextrin concentrations.
Main Methods:
- Enzymatic hydrolysis of icodextrin to glucose using amyloglucosidase.
- Quantification of glucose using a glucose hexokinase assay.
- Calculation of icodextrin concentration by comparing pre- and post-hydrolysis glucose levels.
Main Results:
- The assay demonstrated high recovery rates (100% +/- 15%) in plasma and dialysate.
- The method showed good precision with %RSD within 6% for most concentrations.
- Anion exchange chromatography confirmed complete hydrolysis of icodextrin.
Conclusions:
- A simple, rapid, and reliable enzymatic assay for icodextrin has been developed.
- This assay facilitates routine analysis of icodextrin in plasma and dialysate.
- The method supports effective patient management in peritoneal dialysis.