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[Lipids of Aureobasidium (Pullularia) pullulans]
Abstract:
Fractional composition of free and bound lipids was studied in Aureobasidium (Pullularia) pullulans 8 by preparative TLC on Silufol. Bound lipids contained a fraction (27.76 +/- 0.5%) of dark brown colour, similar to melanin. The composition of fatty acids was studied by GLC. The following fatty acids were identified and determined quantitatively: C12:0, C14:0, C15:0, C16:0, C18:0, C18:1+C15:2. The following fatty acids predominated in free and bound lipids: C16:0, C18:1+C18:2. The ratio between unsaturated and saturated fatty acids in all fractions of free and bound lipids was more than unity. The following parameters were determined for lipids; ester number (173.89 and 178.53); iodine number (44.1 and 33.10), and saponification number (181.17 and 206.03) (the values are given for free and bound lipids, respectively).
Insights
This study analyzed the lipid composition of Aureobasidium pullulans, identifying key fatty acids and characterizing free and bound lipid fractions. The findings reveal a predominance of C16:0 and C18:1+C18:2 fatty acids.
Area of Science:
- Biochemistry
- Microbiology
- Mycology
Context:
- Lipid profiles are crucial for understanding microbial physiology and potential applications.
- Aureobasidium (Pullularia) pullulans is a yeast with diverse metabolic capabilities.
- Characterizing microbial lipids aids in identifying novel bioactive compounds.
Purpose:
- To determine the fractional composition of free and bound lipids in Aureobasidium (Pullularia) pullulans.
- To identify and quantify the fatty acid composition of these lipid fractions.
- To analyze key lipid parameters such as ester, iodine, and saponification numbers.
Summary:
- Preparative Thin-Layer Chromatography (TLC) and Gas-Liquid Chromatography (GLC) were employed to analyze lipids from Aureobasidium (Pullularia) pullulans.
- Bound lipids contained a significant dark brown fraction (27.76 ± 0.5%) resembling melanin.
- Predominant fatty acids in both free and bound lipids were C16:0 and C18:1+C18:2, with unsaturated fatty acids exceeding saturated ones.
Impact:
- Provides detailed lipidomic data for Aureobasidium (Pullularia) pullulans.
- Highlights a potential melanin-like compound within the bound lipid fraction.
- Offers insights into the fatty acid profiles relevant for biotechnological or industrial applications.