Simultaneous determination of dexamethasone and trimethoprim by liquid chromatography

A reverse phase high performance liquid chromatography (HPLC) method to determine dexamethasone phosphate and trimethoprim is described in this paper. The separation was made in a LichrCART(R) C18 column using an acetonitrile-NaH(2)PO(4) (10 mM) (70:30, v/v) (pH 3) buffer solution as mobile phase. The mobile-phase flow rate and the sample volume injected were 1 ml/min and 20 microliter, respectively. The limits of quantification were about 0.25 mg/l for each compound. The method was applied in synthetic mixtures and in pharmaceutical formulations. Analyses were made by preparing test (from the stock solution) method whose results were compared with those obtained by means of the standard addition method. Both methods showed similar results, and then it was proved that some pharmaceuticals claimed levels were in agreement with the obtained results by using our analytical method.