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Updated: Oct 4, 2026

Quantification of the Immunosuppressant Tacrolimus on Dried Blood Spots Using LC-MS/MS
Published on: November 8, 2015
Micellar electrokinetic capillary chromatography as an alternative method for the determination of dexamethasone,
J M Lemus Gallego1, J Perez Arroyo
1Department of Analytical Chemistry and Food Technology, Facultad de Química, Universidad de Castilla-La Mancha, Ciudad Real, Spain. jmlemus@qata-cr.uclm.es
Abstract:
A micellar electrokinetic capillary chromatographic method is presented which enables quantification of dexamethasone, polymyxin B and trimethoprim in synthetic mixtures and pharmaceutical products. Separation was carried out at 25 degrees C and 30 kV, with 10 mmol L(-1) borate-phosphate buffer adjusted to pH 8 as electrolyte, with 50 mmol L(-1) sodium dodecyl sulfate. Under these conditions separations were performed in 10 min. The limits of detection and quantification were approximately 2 mg L(-1) for each component, except for polymyxin B. The method was applied to different commercial formulations.
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