Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A versatile communication module for controlling RNA folding and catalysis.

Alexis Kertsburg1, Garrett A Soukup

  • 1Department of Biomedical Sciences, Creighton University, 2500 California Plaza, Omaha, NE 68178, USA.

Nucleic Acids Research
|November 1, 2002
PubMed
Summary

Researchers discovered a versatile 9-nucleotide RNA element that acts as a communication module, linking different RNA folding domains. This novel element enables effector-controlled RNA function and can identify sites for effector regulation in large RNAs.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Sensational MicroRNAs: Neurosensory Roles of the MicroRNA-183 Family.

Molecular neurobiology·2019
Same author

A mouse model of miR-96, miR-182 and miR-183 misexpression implicates miRNAs in cochlear cell fate and homeostasis.

Scientific reports·2018
Same author

Transcriptome-wide comparison of the impact of Atoh1 and miR-183 family on pluripotent stem cells and multipotent otic progenitor cells.

PloS one·2017
Same author

Identifying microRNAs involved in aging of the lateral wall of the cochlear duct.

PloS one·2014
Same author

Conditional and target-specific transgene induction through RNA replacement using an allosteric trans-splicing ribozyme.

ACS chemical biology·2014
Same author

Characterization of transcriptomes of cochlear inner and outer hair cells.

The Journal of neuroscience : the official journal of the Society for Neuroscience·2014

Area of Science:

  • Molecular Biology
  • Biochemistry
  • RNA Therapeutics

Background:

  • Allosteric ribozymes are engineered for controlled RNA folding and catalysis.
  • Existing communication modules have limitations in versatility.

Purpose of the Study:

  • To isolate and characterize a novel RNA element functioning as a versatile communication module.
  • To demonstrate the element's ability to link disparate RNA folding domains and enable effector regulation.

Main Methods:

  • Isolation and characterization of a novel RNA element.
  • Testing the element's function with various ribozymes (Hepatitis Delta Virus, hammerhead, X motif, Tetrahymena group I) and ligand-binding domains.
  • Mechanistic modeling of RNA folding.

Related Experiment Videos

Main Results:

  • A novel 9-nucleotide RNA element was identified as a versatile communication module.
  • This element functions across diverse ribozyme catalysts and ligand-binding domains.
  • A mechanistic model involving canonical/non-canonical base pairs and an unpaired nucleotide in the effector-bound state was supported.

Conclusions:

  • The novel RNA element enables effector-controlled RNA function through rational design.
  • It serves as a tool to identify sites for effector regulation in large RNA molecules.
  • This discovery advances the engineering of allosteric ribozymes and RNA-based therapeutics.