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Updated: Aug 9, 2026
Split-Ubiquitin Based Membrane Yeast Two-Hybrid (MYTH) System: A Powerful Tool For Identifying Protein-Protein Interactions
Published on: February 1, 2010
Analysis of membrane protein interactions using yeast-based technologies
Igor Stagljar1, Stanley Fields
1Institute of Veterinary Biochemistry and Molecular Biology, University of Zurich-Irchel, Winterthurerstrasse 190, Switzerland. stagljar@vetbio.unizh.ch
Abstract:
Proteins associated with membranes total approximately a third of all proteins in a typical eukaryotic cell. However, the analysis of interactions between membrane proteins is difficult because of the hydrophobic nature of these proteins, and conventional biochemical and genetic assays are often of limited use. We summarize here recent yeast-based interaction technologies that can be applied to membrane proteins.
