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Oligonucleotide microarray data mining: search for age-dependent gene expression.

Marc Kirschner1, Gemma Pujol, Aurelian Radu

  • 1Carl C. Icahn Institute for Gene Therapy and Molecular Medicine, Mount Sinai School of Medicine, New York, NY 10029, USA.

Biochemical and Biophysical Research Communications
|November 7, 2002
PubMed
Summary
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Gene expression in colon tumors and normal tissues shows age-dependent variations. Some p53-controlled genes and ribosomal genes exhibit changes with age, particularly in tumor tissues.

Area of Science:

  • Genomics
  • Molecular Biology
  • Cancer Research

Background:

  • Oligonucleotide microarrays provide gene expression data for colon tumors and normal colon tissues.
  • Age-dependent variations in gene expression are not fully understood in the context of aging and cancer.
  • Previous studies have not comprehensively analyzed age-related gene expression changes across different functional gene subsets.

Purpose of the Study:

  • To investigate age-dependent gene expression variations in both colon tumor and normal colon tissues.
  • To identify specific gene groups, including those regulated by p53, ribosomal proteins, and mitochondrial proteins, that are associated with age.
  • To determine if age-related expression patterns differ between cancerous and healthy colon tissues.

Main Methods:

Related Experiment Videos

  • Utilized an existing oligonucleotide microarray database of gene expression in colon tumors and normal human colon.
  • Performed statistical analysis to identify genes with significant age-dependent expression changes.
  • Analyzed specific gene subsets: p53-controlled genes, ribosomal protein genes, and nuclear-encoded mitochondrial protein genes.

    • Main Results:

    • Confirmed the presence of statistically significant age-dependent gene expression variations in both tumor and normal colon tissue datasets.
    • Observed age-related expression changes in some p53-controlled genes within tumor tissues, suggesting potential p53 activation with age.
    • Detected decreased expression of certain ribosomal genes in advanced age, evident in both tumor and normal tissues.
    • Found no significant age-dependent expression trends for nuclear-encoded mitochondrial protein genes.

    Conclusions:

    • Age significantly influences gene expression patterns in both colon tumors and normal colon tissues.
    • Specific gene groups, such as those regulated by p53 and ribosomal proteins, show age-related alterations, with implications for cancer development and aging.
    • The findings highlight the complex interplay between aging, gene regulation, and colon cancer, underscoring the need for further research into these mechanisms.