Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Voltage-sensitive dye mapping in Langendorff-perfused rat hearts.

A Nygren1, C Kondo, R B Clark

  • 1Department of Physiology and Biophysics, University of Calgary, Calgary, Canada T2N 4N1.

American Journal of Physiology. Heart and Circulatory Physiology
|November 9, 2002
PubMed
Summary

A new imaging system for rat hearts using voltage-sensitive dye (di-4-ANEPPS) was developed. It revealed that altered potassium levels and temperature significantly impact cardiac conduction velocity and activation patterns.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Treatment Strategies and Effectiveness in Lymphatic Malformations: A 10-Year Retrospective Study.

Lymphology·2025
Same author

Two apparently unrelated groups of symbiotic annelids, Nautiliniellidae and Calamyzidae (Phyllodocida, Annelida), are a clade of derived chrysopetalid polychaetes.

Cladistics : the international journal of the Willi Hennig Society·2021
Same author

Improved Accuracy of Cardiac Tissue-Level Simulations by Considering Membrane Resistance as a Cellular-Level Optimization Objective.

Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference·2020
Same author

A Novel Bi-Level Framework for Fitting the Parameters in Cardiac Cellular Models.

Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference·2018
Same author

ATP triggers a robust intracellular [Ca<sup>2+</sup> ]-mediated signalling pathway in human synovial fibroblasts.

Experimental physiology·2018
Same author

Arrhythmia Vulnerability in Diabetic Cardiac Tissue is Species-Dependent: Effects of I <sub>KATP</sub>, Uncoupling, and Connexin Lateralization.

Cardiovascular engineering and technology·2017

Area of Science:

  • Cardiovascular Physiology
  • Biomedical Imaging
  • Electrophysiology

Background:

  • Voltage-sensitive dyes are crucial for optical mapping of cardiac electrical activity.
  • Langendorff-perfused rat hearts provide a controlled model for studying cardiac function.
  • Understanding factors affecting cardiac conduction is vital for diagnosing and treating arrhythmias.

Purpose of the Study:

  • To develop and validate an imaging system for voltage-sensitive dye recordings in Langendorff-perfused rat hearts.
  • To investigate the effects of ionic and temperature changes on cardiac conduction velocity and activation patterns.
  • To characterize the impact of mechanical stabilization and dye application on cardiac electrophysiology.

Main Methods:

  • Development of an imaging system for voltage-sensitive dye (di-4-ANEPPS) recordings.

Related Experiment Videos

  • Perfusion of isolated rat hearts using the Langendorff technique.
  • Measurement of conduction velocity under varying extracellular potassium concentrations and temperatures.
  • Analysis of ventricular activation patterns during sinus and paced rhythms.
  • Assessment of mechanical immobilization and dye-induced artifacts.

    • Main Results:

    • Hyperkalemia (9 mM K+) slowed conduction velocity by 35% (0.66 to 0.43 mm/ms).
    • Hypothermia (32°C) slowed conduction velocity by 28% (0.64 to 0.46 mm/ms).
    • Ventricular activation patterns exhibited early activation sites (breakthrough), typically originating near the right ventricular apex.
    • Mechanical immobilization affected sinus rhythm activation patterns and prolonged PQ intervals, but not paced rhythms.
    • Dye perfusion caused transient vasodilation and prolonged PQ intervals, sometimes leading to atrioventricular block.

    Conclusions:

    • The developed imaging system is effective for studying cardiac electrophysiology in rat hearts.
    • Extracellular potassium levels and temperature significantly modulate cardiac conduction velocity.
    • Mechanical stabilization and dye application can introduce artifacts, requiring careful consideration.
    • The study provides insights into normal and altered cardiac electrical propagation under physiological and pathological conditions.