Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Pyrazolylborate-zinc-nucleobase-complexes, 3:(1) base pairing studies.

Dirk Badura1, Heinrich Vahrenkamp

  • 1Institut für Anorganische und Analytische Chemie der Universität Freiburg, Albertstr. 21, D-79104 Freiburg, Germany.

Inorganic Chemistry
|November 12, 2002
PubMed
Summary

Pyrazolylborate-zinc complexes exhibit self-association and form base pairs with nucleobases in solution. These complexes demonstrate specific interactions, leading to stable base pairing in both solution and solid states.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Why does nature use zinc--a personal view.

Dalton transactions (Cambridge, England : 2003)·2007
Same author

Modeling zinc enzyme inhibition with functional thiolate ligands.

Inorganic chemistry·2006
Same author

Zn-OH2 and Zn-OH complexes with hydroborate-derived tripod ligands: a comprehensive study.

Inorganic chemistry·2006
Same author

Thiolate alkylation in tripod zinc complexes: a comparative kinetic study.

Inorganic chemistry·2006
Same author

Tris(thioimidazolyl)borate-zinc-thiolate complexes for the modeling of biological thiolate alkylations.

Inorganic chemistry·2005
Same author

Reactions of pyrazolylborate-zinc-hydroxide complexes related to beta-lactamase activity.

Inorganic chemistry·2005

Area of Science:

  • Coordination Chemistry
  • Supramolecular Chemistry
  • Nucleobase Recognition

Background:

  • Pyrazolylborate ligands are versatile in coordinating metal ions.
  • Nucleobase pairing is fundamental in biological systems and supramolecular assembly.
  • Understanding metal-nucleobase interactions is key to designing novel functional materials.

Purpose of the Study:

  • To investigate the self-association and external nucleobase pairing of pyrazolylborate-zinc complexes.
  • To quantify the dimerization constants and association constants of these interactions.
  • To elucidate the structural basis of base pairing in both solution and solid states.

Main Methods:

  • Solution-state NMR spectroscopy to determine dimerization and association constants.

Related Experiment Videos

  • X-ray crystallography to determine the solid-state structures of crystalline adducts.
  • Spectroscopic analysis of pyrazolylborate-zinc-nucleobase complexes.

    • Main Results:

    • Quantified self-association for Tp(Cum,Me)Zn-hypoxanthinate (K(D) = 63 +/- 8 M(-1)) and Tp(Cum,Me)Zn-thyminate (K(D) = 0.2 +/- 0.1 M(-1)).
    • Identified specific nucleobase pairings, such as 9-ethyladenine with thyminate, uracilate, and xanthinate complexes.
    • Confirmed Watson-Crick and Hoogsteen base pairing in crystalline adducts, with extended arrangements in the solid state.

    Conclusions:

    • Pyrazolylborate-zinc complexes display tunable self-association and selective nucleobase pairing capabilities.
    • These interactions are structurally characterized, revealing diverse base pairing motifs.
    • The findings contribute to the understanding of non-covalent interactions in coordination chemistry and supramolecular assembly.