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A method for estimation of urease activity in gastric mucosa biopsy specimens and Helicobacter pylori cell
N O Vartanova1, V G Arzumanyan, I A Basnak'yan
1I. I. Metchnikoff Institute of Vaccines and Sera, Russian Academy of Medical Sciences, Moscow.
Abstract:
A method for measuring urease activity in biopsy specimens and Helicobacter pylori cultures from these specimens is proposed. The method is based on measurement (with a portable pH-meter) of the rate of pH changes in a reaction mixture consisting of buffer, substrate (urea), and biopsy specimen or bacterial cells. This method revealed that urease activity of biopsy specimens correlated with that of H. pylori suspension in the same experiment. High urease activity was found in biopsy specimens containing the greatest number of Helicobacter cells; only one of 14 specimens free of H. pylori cells showed no urease activity. Introduction of this method into clinical practice will help to evaluate the contribution of H. pylori to the pathological process.
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