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Related Experiment Videos

Cold-shock and the Mammalian cell cycle.

Conly L Rieder1, Richard W Cole

  • 1Laboratory of Cell Regulation, Division of Molecular Medicine, Wadsworth Center; New York State Department of Health; Albany, New York 12201-0509 USA. Reider@wadsworth.org

Cell Cycle (Georgetown, Tex.)
|November 14, 2002
PubMed
Summary

Cell cycle progression is temperature-sensitive. Moderate hypothermia can enrich for G(2) phase cells, while severe hypothermia may induce mitotic synchrony in mammalian cultures.

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Area of Science:

  • Cell Biology
  • Mammalian Cell Cycle Regulation
  • Temperature Sensitivity

Background:

  • Cell cycle progression is influenced by temperature, but the relationship is complex.
  • Mammalian cells often arrest at the G(2)/M transition when cooled to moderate hypothermia (16-20°C).
  • Progression through G(1), S, and mitosis is less sensitive to moderate hypothermia.

Purpose of the Study:

  • To investigate the temperature sensitivity of cell cycle progression in mammalian cells.
  • To explore the potential of hypothermia for cell cycle synchronization and enrichment.
  • To understand the specific cell cycle phases affected by temperature shifts.

Main Methods:

  • Culturing mammalian cells at different temperatures (37°C, 16-20°C, 4-10°C).

Related Experiment Videos

  • Analyzing cell cycle progression through different phases (G(1), S, G(2), M).
  • Assessing cell cycle synchrony and enrichment after hypothermic exposure.

    • Main Results:

    • Moderate hypothermia (16-20°C) selectively disrupts a G(2) phase pathway, preventing G(2)/M transition.
    • Prolonged moderate hypothermia (24-48 hr) effectively enriches cell cultures for G(2) phase cells.
    • Brief severe hypothermia (4-10°C) can induce significant mitotic synchrony (up to 80%) in some cell types.
    • The mechanism for severe hypothermia-induced synchrony may involve a cold shock-triggered cell cycle checkpoint inhibiting G(1)/S transition.

    Conclusions:

    • Temperature plays a critical role in regulating specific cell cycle transitions, particularly the G(2)/M phase.
    • Hypothermia, applied strategically, can be a valuable tool for cell cycle synchronization and G(2) cell enrichment in research.
    • Further investigation is needed to elucidate the precise molecular mechanisms underlying hypothermia-induced cell cycle arrest and synchrony.