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Related Experiment Videos

Arachidonate 8(S)-lipoxygenase.

Gerhard Fürstenberger1, Friedrich Marks, Peter Krieg

  • 1Research Program Tumor Cell Regulation, Deutsches Krebsforschungszentrum, Heidelberg, Germany. G.Furstenberger@dkfz-heidelberg.de

Prostaglandins & Other Lipid Mediators
|November 16, 2002
PubMed
Summary

Mouse 8(S)-lipoxygenase, a homolog of human 15(S)-lipoxygenase-2, is crucial for skin cell differentiation and tumor progression. Its expression in mouse epidermis highlights its role in keratinocyte terminal differentiation.

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Dermatology

Background:

  • Mouse 8(S)-lipoxygenase (Alox15b) exhibits specific oxygenation of fatty acids, showing high identity to human 15(S)-lipoxygenase-2.
  • The Alox15b gene is located on mouse chromosome 11 within a cluster of epidermis-type lipoxygenase genes.

Purpose of the Study:

  • To characterize the mouse 8(S)-lipoxygenase, including its enzymatic activity, gene location, expression patterns, and role in epidermal differentiation and skin tumorigenesis.

Main Methods:

  • Sequence analysis to determine homology with human lipoxygenases.
  • Gene mapping to identify the chromosomal location of Alox15b.
  • Expression analysis in various mouse tissues and skin layers.
  • Investigation of enzyme activity in relation to keratinocyte differentiation and tumor development.

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Main Results:

  • Mouse 8(S)-lipoxygenase primarily oxygenates arachidonic acid at the 8(S) position and linoleic acid at the 9(S) position.
  • The enzyme is predominantly expressed in terminally differentiating keratinocytes of the mouse epidermis, particularly in the stratum granulosum.
  • Expression is constitutive in hair follicles, forestomach, and foot-sole, and inducible in back skin.
  • Tumor-specific upregulation of 8(S)-lipoxygenase suggests a role in malignant progression.

Conclusions:

  • Mouse 8(S)-lipoxygenase plays a significant role in the terminal differentiation of mouse epidermis.
  • The enzyme's activity and expression are implicated in the malignant progression of skin tumors in mice.