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[Stability of genetic markers in methane-oxidizing bacteria]
V A Romanovskaia1, P V Rokitko, Iu R Malashenko
1Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, 154 Zabolotny St., Kyiv, 03143, Ukraine.
Mikrobiolohichnyi Zhurnal (Kiev, Ukraine : 1993)
|November 20, 2002
Summary
Chromosomal antibiotic resistance markers are stable in Methylococcus thermophilus and Methylomonas rubra, enabling their use in selective media. Plasmid-based markers showed less stability in these methane-oxidizing bacteria.
Area of Science:
- Microbiology
- Bacterial Genetics
Background:
- Investigating antibiotic resistance marker stability in methane-oxidizing bacteria like Methylococcus thermophilus and Methylomonas rubra is crucial for their application in biotechnology.
- Understanding the inheritance patterns of chromosomal versus plasmid-borne resistance markers provides insights into bacterial genetic stability.
Purpose of the Study:
- To assess the stability of antibiotic and formaldehyde resistance markers in Methylococcus thermophilus 111p and Methylomonas rubra 15sh mutants.
- To determine if these marked strains can be reliably used in laboratory and industrial settings.
Main Methods:
- Acquisition of tetracycline resistance in Methylococcus thermophilus 111p.
- Maintenance and stability testing of chromosomal and plasmid-borne resistance markers under nonselective conditions.
- Creation of a collection of marked bacterial strains.
Main Results:
- Chromosomal markers for antibiotic and formaldehyde resistance were stably maintained in Methylococcus thermophilus 111p and Methylomonas rubra 15sh after storage.
- Plasmid-coded antibiotic resistance markers (pAS8-121, pULB113) were not consistently preserved in these bacterial strains.
- The created collection of marked strains demonstrated stable resistance under unselective conditions.
Conclusions:
- Stable chromosomal markers in Methylococcus thermophilus and Methylomonas rubra support their use for testing on selective media.
- The findings highlight the reliability of chromosomal markers over plasmid markers for long-term strain stability in these bacteria.