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Cholesterol oxidation using hollow fiber dialyzer immobilized with cholesterol oxidase: effect of storage and reuse
Chi-Chang Lin1, Ming-Chien Yang
1Department of Polymer Engineering, Hollow Fiber Laboratory, National Taiwan University of Science and Technology, Taipei 10672, Taiwan.
Abstract:
Cholesterol oxidase (COD) was covalently bonded onto the surface of polyacrylonitrile (PAN) hollow fiber via glutaraldehyde. The optimum conditions were found to be at pH 7, -20 degrees C and in the dry state. Immobilized COD retained relative activity above 42% after a 30-day period, when storing at 25 degrees C. After reusing for 30 times, the activity of dry-stored immobilized COD retained 58% of activity. By rinsing with PBS, the decrease in the activity can be greatly reduced. At a flow speed of 1.09cm/s, the immobilized COD can decrease the concentration of cholesterol by about 40% in a 4-h run.
