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Related Experiment Videos

Decrease in cell viability in an RMF, sigma(38), and OmpC triple mutant of Escherichia coli.

V Samuel Raj1, Christine Füll, Madoka Yoshida

  • 1Graduate School of Pharmaceutical Sciences, Chiba University, 1-33 Yayoi-cho, Inage-ku, 263-8522, Chiba, Japan.

Biochemical and Biophysical Research Communications
|November 20, 2002
PubMed
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Spermidine negatively impacts Escherichia coli viability in late stationary phase. Cooperative functions of ribosome modulation factor (RMF), sigma(38) (encoded by rpoS), and outer membrane protein C (OmpC) are crucial for cell survival.

Area of Science:

  • Microbiology
  • Bacterial Physiology
  • Molecular Biology

Background:

  • Spermidine metabolism is essential for bacterial growth.
  • Stationary phase survival is critical for bacterial populations.
  • Specific proteins like RMF, sigma(38), and OmpC play roles in bacterial stress response.

Purpose of the Study:

  • To investigate the effect of spermidine on Escherichia coli viability.
  • To determine the roles of ribosome modulation factor (RMF), sigma(38) (encoded by rpoS), and outer membrane protein C (OmpC) in cell viability.
  • To elucidate the cooperative functions of these proteins in late stationary phase survival.

Main Methods:

  • Utilized a speG-disrupted Escherichia coli mutant unable to metabolize spermidine.
  • Assessed cell viability upon spermidine addition.

Related Experiment Videos

  • Generated single, double, and triple gene disruptants for rmf, rpoS, and ompC.
  • Quantified protein levels and ribosome/cation content.

    • Main Results:

    • Spermidine addition decreased cell viability in speG mutant.
    • Levels of RMF, sigma(38), and OmpC decreased in parallel.
    • Triple rmf, rpoS, and ompC mutants exhibited significantly reduced viability (<1%).
    • Cooperative function of RMF, sigma(38), and OmpC is essential for late stationary phase viability.
    • Triple mutants showed reduced ribosomes and intracellular cations.

    Conclusions:

    • Cooperative action of RMF, sigma(38), and OmpC is vital for Escherichia coli survival.
    • Disruption of these proteins impairs ribosome and cation homeostasis.
    • Spermidine's role in regulating stationary phase viability is mediated through these proteins.