Related Experiment Videos
Hypoxia activates multiple transcriptional pathways in mouse pheochromocytoma cells
Marian J Evinger1, Stefan Cikos, Victoria Nwafor-Anene
1Department of Pediatrics, State University of New York at Stony Brook, Stony Brook, New York 11794, USA. mevinger@notes.cc.sunysb.edu
Abstract:
Mouse pheochromocytoma cells (MPCs) provide an excellent model system for investigating the effects of hypoxia on catecholamine enzyme genes and on transcription factors mediating stress responses. RT-PCR detects rapid, transient increases in PNMT mRNA in hypoxic MPC 712 cells. Additionally, elevation of mRNAs encoding transcription factors hypoxia inducible factor 1 (HIF-1) alpha subunit and Egr-1 are evident within 60 min incubation in anoxia. Therefore, hypoxia elicits rapid transcriptional responses in numerous genes expressed by chromaffin cells.