Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Gene expression studies using microarrays: principles, problems, and prospects.

David Murphy1

  • 1University of Bristol Research Centre for Neuroendocrinology, Bristol Royal Infirmary, Bristol BS2 8HW, England. d.murphy@bristol.ac.uk

Advances in Physiology Education
|November 22, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Ageing Adipose Paradox: Implications for Metabolic Health.

Diabetes, obesity & metabolism·2026
Same author

Assessing the impact of combined nutritional and physical activity interventions for patients with atrial fibrillation who are overweight: a systematic review protocol.

BMJ open·2026
Same author

Diagnosing autism in adult forensic settings.

Medicine, science, and the law·2026
Same author

Arginine vasopressin: A promising therapeutic target for metabolic syndrome.

The Journal of pharmacology and experimental therapeutics·2026
Same author

Beyond the gut: a case report of <i>Shigella</i>-associated myocarditis.

European heart journal. Case reports·2026
Same author

Improving the Understanding of Late Effects of Testicular Cancer in Adolescent and Young Adult Survivors: TRANSCEND-XR.

Andrology·2026
Same journal

Medical Physiology Faculty Overpredict Assessment Question Complexity and Alignment with National Board Expectations.

Advances in physiology education·2026
Same journal

Correction for Kamel-ElSayed and Uhley, volume 48, 2024, p. 648-654.

Advances in physiology education·2026
Same journal

Cellular and network mechanisms of spreading depolarization.

Advances in physiology education·2026
Same journal

Figuring It Out: How I Became a Decent Anatomy Teacher in Three Hard Steps.

Advances in physiology education·2026
Same journal

A Low-Cost Metabolic Apparatus for Student Labs: Measuring Oxygen Consumption and Ventilation in Conscious Mice.

Advances in physiology education·2026
Same journal

Teaching Thyroid Physiology Through Art: An Integrative And Historical Pedagogical Approach.

Advances in physiology education·2026
See all related articles

Microarray technology is crucial for understanding gene networks in health and disease. This review details microarray experiments, addressing challenges and comparing techniques for accurate gene expression profiling.

Area of Science:

  • Genomics
  • Molecular Biology
  • Bioinformatics

Background:

  • Mammalian genome sequencing provides a foundation for large-scale gene expression studies.
  • Understanding gene networks is essential for deciphering developmental, physiological, and pathological processes.
  • Microarray technology offers a powerful tool for cataloging transcription unit expression patterns.

Purpose of the Study:

  • To review the stages of a microarray experiment.
  • To compare advantages and disadvantages of different techniques used in microarrays.
  • To identify and discuss sources of error in microarray experiments.

Main Methods:

  • The review covers RNA isolation, target preparation, and hybridization.
  • It includes data capture and bioinformatic analysis stages.

Related Experiment Videos

  • Comparative analysis of competing techniques is presented.

    • Main Results:

    • Microarray technology is rapidly evolving with evolving methodologies and standards.
    • Experimental design and variability present significant challenges.
    • Each stage of the microarray process has inherent sources of error.

    Conclusions:

    • Despite challenges, microarray technology is vital for gene expression cataloging.
    • Addressing technical and analytical issues is key to realizing the full potential of microarrays.
    • Standardization and understanding variability are crucial for reliable gene expression data.