Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Integrating interactome, phenome, and transcriptome mapping data for the C. elegans germline.

Albertha J M Walhout1, Jérôme Reboul, Olena Shtanko

  • 1Dana-Farber Cancer Institute and Department of Genetics, Harvard Medical School, Boston, MA 02115, USA.

Current Biology : CB
|November 26, 2002
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Metamorphosis of the purple sea urchin Heliocidaris crassispina: Single-cell transcriptomic datasets.

Scientific data·2026
Same author

Genome evolution in parthenogenetic nematodes shaped by chromosome rearrangements and introgression.

Genome research·2026
Same author

An Interdisciplinary Course Development in Nursing Informatics: A Hybrid Teaching Model.

Computers, informatics, nursing : CIN·2026
Same author

Characterization and genomic analysis of a novel Vibrio harveyi Vibrio phage LRZ.

Archives of virology·2026
Same author

Bacteria producing the bioplastic polyhydroxybutyrate kill the nematode Caenorhabditis elegans.

PLoS biology·2026
Same author

Environmental Responses and Interspecific Associations of Fish Communities in the Zhoushan Fishing Ground Revealed by HMSC.

Animals : an open access journal from MDPI·2026
This summary is machine-generated.

Integrating interactome, phenome, and transcriptome data reveals key germline biology in C. elegans. Essential proteins interact and share expression profiles, with 24% of interactions showing linked phenotypes.

Area of Science:

  • Genomics and Proteomics
  • Systems Biology
  • Developmental Biology

Background:

  • Functional genomic and proteomic mapping are crucial for generating robust biological hypotheses.
  • Integrating diverse datasets like interactome, phenome, and transcriptome data enhances biological insight.

Purpose of the Study:

  • To integrate interactome, phenome, and transcriptome data for the Caenorhabditis elegans germline.
  • To identify biologically relevant interactions within the C. elegans germline.

Main Methods:

  • Generated a two-hybrid interactome map using 600 enriched C. elegans germline transcripts.
  • Compared interactome data with a germline phenome map (RNA interference) and transcriptome map (gene expression profiling).

Main Results:

Related Experiment Videos

  • Essential proteins tend to interact with each other.
  • Interacting protein pairs often exhibit similar gene expression profiles.
  • Approximately 24% of germline interactions involve partners with overlapping RNAi phenotypes and similar expression profiles.

Conclusions:

  • These integrated data suggest specific interactions are highly relevant to germline biology.
  • This integrative approach is applicable to other biological processes and organisms, including humans.