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Possible use of established cell lines for MLR locus typing
Tissue Antigens
|April 1, 1975
Summary
Established lymphoblastoid cell lines can replace fresh lymphocytes for mixed lymphocyte reaction (MLR) histocompatibility typing. This finding supports using cell lines to standardize MLR typing and improve donor selection for transplantation.
Area of Science:
- Immunogenetics
- Transplantation immunology
Background:
- Mixed lymphocyte reaction (MLR) is crucial for histocompatibility typing.
- Current MLR typing relies on fresh lymphocytes, which can be limiting.
Purpose of the Study:
- To evaluate the utility of lymphoblastoid cell lines (LCLs) as substitutes for fresh lymphocytes in MLR histocompatibility typing.
- To determine if LCLs can accurately reflect MLR locus products.
Main Methods:
- In vitro stimulation of lymphocytes from various individuals using irradiated fresh lymphocytes or mitomycin-C-treated LCLs derived from homozygous MLR donors.
- Correlation analysis of MLR responses between fresh lymphocyte and LCL stimulation.
Main Results:
- MLC responses showed a strong correlation when stimulated by either fresh lymphocytes or LCLs.
- Established LCLs effectively express MLR locus products.
- Long-term cell lines can substitute for fresh lymphocytes in MLR typing.
Conclusions:
- Lymphoblastoid cell lines are viable alternatives to fresh lymphocytes for MLR histocompatibility typing.
- Development of a panel of LCLs from homozygous donors could standardize MLR typing.
- This approach may facilitate the selection of histocompatible unrelated donors for transplantation.