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[Chemical constituents analysis on anticonvulsive effect of three extracts from radix bupleuri]
Objective:
To study the relationship between anticonvulsive effect of three extracts from Radix Bupleuri and their chemical compositions.
Methods:
Volatile oil component, saikosaponin component and water-soluble component were extracted with SFE-CO2 from Radix Bupleuri, their effect on MES and PTZ seizure models had been studied, and their compositions were analyzed by GC-MS and HPLC.
Results:
Compared with the control, volatile oil component and saikosaponin component significantly decreased anticonvulsive occurrence on MES, the main chemical compositions of them was linoleic acid; water-soluble component significantly decreased anticonvulsive occurrence on PTZ seizure, but most of its compositions were glycerol and xylitol.
Conclusion:
Linoleic acid from Radix Bupleuri maybe fight against MES seizure, while glycerol and xylitol could fight against PTZ seizure.