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Updated: Aug 23, 2026
Detection of Mitochondria Membrane Potential to Study CLIC4 Knockdown-induced HN4 Cell Apoptosis In Vitro
Published on: July 17, 2018
[Clotrimazole induced apoptosis in human colon cancer cell line CCL229]
1Key Laboratory of Cell Biology, Ministry of Public Health of China, China Medical Unviersity, Shenyang 110001, P. R. China.
Background & Objective:
It was reported that clotrimazole could inhibit the growth of tumor cell by exhausting cellular calcium. This study was designed to investigate whether clotrimazole(CLT) can induce apoptosis invitro in human colon cancer cell line CCL229.
Methods:
The fluorescent microscope, electron microscopes, flow cytometry and terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling(TUNEL) were applied to detect the morphological changes, cells cycle distribution, and DNA fragmentation.
Results:
Apoptosis can be induced in colon cancer cell CCL229 by CLT at the concentration of 25-35 mumol/L in a dose and time dependent manner. Typical morphological changes of apoptosis such as apoptic body were found under fluorescent microscope and electron microscopes. DNA histograms showed the hypodiploid peak, the ratio of hypodiploid cells was increased with the increase of dose and elongation of time. The ration of cells in G1 phase decrease accordingly. Brown-colored positive apoptotic cells were observed with TUNEL method.
Conclusion:
CLT can induce apoptosis in vitro in human colon cancer cell line CCL 229. The apoptosis inducing effect may be G1 phase-specific.
