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Updated: Jul 17, 2026

Directed Protein Packaging within Outer Membrane Vesicles from Escherichia coli: Design, Production and Purification
Published on: November 16, 2016
Surface-bound lipid vesicles encapsulating redox species for amperometric biosensing of pore-forming bacterial toxins
1Department of Chemistry, University of California, Riverside, California 92521, USA.
Abstract:
A new method is described to signal the presence of a staphylococcal toxin (streptolysin, SLO) by a membrane-based biosensor fabricated with bilayer vesicles of phosphatidylcholine lipids and cholesterol on a gold electrode. The controlled release of encapsulated redox species from the surface-bound vesicles is mediated by the pore-formation functionality of SLO, allowing amperometric detection of the targeted toxin. Effects of BSA and Triton-100 on membrane permeability and redox release have been assessed, and a thin layer electrolysis mechanism has been proposed to account for the observed voltammetric behavior. The method is applicable to the detection of functionally similar protein toxins.
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