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Gene expression profiles in chondrosarcoma cells subjected to cyclic stretching and hydrostatic pressure. A cDNA
Hannu M Karjalainen1, Reijo K Sironen, Mika A Elo
1Department of Anatomy, University of Kuopio, P.O. Box 1627, 70211 Kuopio, Finland. mikko.lammi@uku.fi
Biorheology
|November 28, 2002
Summary
Mechanical forces impact cartilage cells. Cyclic stretch significantly altered gene expression in chondrosarcoma cells more than hydrostatic pressure, affecting cell cycle and matrix genes.
Area of Science:
- Biomedical Engineering
- Cell Biology
- Molecular Biology
Background:
- Mechanical forces profoundly influence cartilage tissue and chondrocyte metabolism.
- Optimal loading promotes an anabolic response, while strenuous loading inhibits cellular metabolism.
Purpose of the Study:
- To investigate mRNA expression profiles in human chondrosarcoma cells subjected to mechanical stimuli.
- To compare the effects of cyclic stretch and hydrostatic pressure on gene expression.
Main Methods:
- Human chondrosarcoma cells were subjected to 8% cyclic stretch at 0.5 Hz for 6 hours.
- Cells were also exposed to continuous and cyclic (0.5 Hz) 5 MPa hydrostatic pressure.
- mRNA expression profiles were analyzed using an Atlas Human Cancer cDNA array.
Main Results:
- Cyclic stretch significantly altered gene expression compared to hydrostatic pressure.
- Stretched cells showed increased expression of cell cycle regulators, PDGF-B, and MMP-3, with decreased expression of plakoglobin and TGF-alpha.
- Hydrostatic pressure induced fewer changes, with increased plakoglobin and decreased MAPK-9 and PCNA.
Conclusions:
- Different mechanical stimuli (cyclic stretch, hydrostatic pressure) elicit distinct gene expression profiles in chondrosarcoma cells.
- Cyclic stretch has a more pronounced effect on gene expression than hydrostatic pressure.
- These findings highlight the differential cellular responses to mechanical loading in cartilage.