Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

In vitro models to study compressive strain-induced muscle cell damage.

Carlijn V C Bouten1, Roel G M Breuls, Emiel A G Peeters

  • 1Department of Biomedical Engineering, Eindhoven University of Technology, PO Box 513, 5600 MB Eindhoven, The Netherlands. C.V.C.Bouten@tue.nl

Biorheology
|November 28, 2002
PubMed
Summary

Sustained muscle cell deformation from compression causes tissue breakdown. Researchers developed in vitro models to study this cellular damage, finding deformation significantly increases with strain over time.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Traction Force Microscopy for Viscoelastic Substrates: A Semi-Analytical Method.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Restoration of structural organization in engineered cardiac microtissues is promoted by cardiomyocyte beating.

Communications biology·2026
Same author

A noncanonical role for Jagged1 in endothelial mechanotransduction.

The FEBS journal·2026
Same author

Mechanosensitive interactions between Jag1 and Myo1c control Jag1 trafficking in endothelial cells.

iScience·2025
Same author

Macrophage Paracrine Signalling Differentially Affects Fibroblast-Induced Collagenous Tissue Remodelling.

Tissue engineering and regenerative medicine·2025
Same author

Substrate stiffness modulates phenotype-dependent fibroblast contractility and migration independent of TGF-<i>β</i> stimulation.

Mechanobiology in medicine·2025

Area of Science:

  • Biomedical Engineering
  • Cellular Biology
  • Tissue Mechanics

Background:

  • Skeletal muscle is vulnerable to pressure-induced damage, like pressure sores.
  • Cellular-level damage is triggered by sustained cell deformation under compressive strain.

Purpose of the Study:

  • Investigate the link between compressive strain, muscle cell deformation, and tissue damage.
  • Explore the roles of cell-cell and cell-matrix interactions, and tissue geometry in this process.
  • Develop in vitro models for studying muscle tissue under compression.

Main Methods:

  • Created in vitro models: single cells, monolayers, and 3D tissue analogs.
  • Applied compression using specialized loading devices.
  • Visualized cell deformation via confocal microscopy.

Related Experiment Videos

  • Assessed cell damage using viability tests, vital microscopy, and histological/biochemical analyses.

    • Main Results:

    • Preliminary data from a 3D cell-seeded agarose model confirms cell deformation as a key damage trigger.
    • Sustained compression at 20% strain significantly increased cell damage over time.
    • Unstrained control groups showed constant damage levels.

    Conclusions:

    • Cell deformation is a critical factor in pressure-induced skeletal muscle damage.
    • In vitro models are effective tools for studying the mechanisms of tissue breakdown under mechanical stress.
    • Further research can refine these models to better understand and prevent pressure sores.