Related Experiment Videos
Cytokine dialysis: an ex vivo study
Shigehiko Uchino1, Rinaldo Bellomo, Hiroshi Morimatsu
1Department of Intensive Care and Medicine, Austin & Repatriation Medical Centre, Melbourne, Victoria, Australia.
ASAIO Journal (American Society for Artificial Internal Organs : 1992)
|November 29, 2002
Summary
New large pore membranes effectively remove cytokines during dialysis, a promising blood purification technique for sepsis. This method achieves significant cytokine clearance with minimal albumin loss, supporting its technical feasibility.
Area of Science:
- Biomedical Engineering
- Renal Physiology
- Immunology
Background:
- Sepsis involves harmful cytokine storms.
- Current blood purification methods have limitations in cytokine removal.
- Novel membranes may enhance extracorporeal cytokine clearance.
Purpose of the Study:
- To evaluate a new large pore polyamide membrane for cytokine removal during hemodialysis.
- To assess the impact of dialysate flow rate on cytokine and albumin clearance.
- To determine the technical feasibility of this approach for sepsis treatment.
Main Methods:
- Blood from six volunteers was incubated with endotoxin.
- Circulation through a closed system with a 100 kDa cut-off polyamide membrane.
- Hemodialysis performed at 1 L/hr and 9 L/hr dialysate flow rates.
Main Results:
- High dialysate/plasma concentration ratios achieved for key cytokines (IL-1beta, IL-6, IL-8, TNF-a).
- Increased dialysate flow significantly improved cytokine clearances (IL-1beta, IL-6, IL-8) and reduced albumin loss.
- Peak clearances at 9 L/hr: IL-1beta (33 ml/min), IL-6 (19 ml/min), IL-8 (51 ml/min).
Conclusions:
- Large pore membrane dialysis effectively removes cytokines.
- This method shows technical feasibility for blood purification in sepsis.
- Negligible albumin loss observed, indicating selectivity.