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Characterisation of reversed-phase liquid chromatographic columns by chromatographic tests. Evaluation of 36 test
Dóra Visky1, Yvan Vander Heyden, Tímea Iványi
1Laboratorium voor Farmaceutische Chemie en Analyse van Geneesmiddelen, Katholieke Universiteit Leuven, Van Evenstraat 4, B-3000 Leaven, Belgium.
Abstract:
The European Pharmacopoeia (Ph. Eur.) or other official compendia give only a general description of the stationary phase in the description of a liquid chromatographic method. Therefore the selection of a column giving suitable selectivity presents difficulties. Earlier, a test procedure was proposed that allows measurement of a number of parameters which are reported to be representative for stationary phase characteristics. This paper describes how the test procedure was applied on 69 RP-LC C18 columns. Chromatographic parameters obtained as test results were evaluated, and their repeatability, reproducibility and correlation were examined.